DNA-free RNA preparations from mycobacteria

Abstract

Background: To understand mycobacterial pathogenesis analysis of gene expression by quantification of RNA levels becomes increasingly important. However, current preparation methods yield mycobacterial RNA that is contaminated with chromosomal DNA. Results: After sonication of RNA samples from Mycobacterium smegmatis genomic DNA is efficiently removed by DNaseI in contrast to untreated samples. Conclusions: This procedure eliminates one of the most prevalent error sources in quantification of RNA levels in mycobacteria. © 2004 Stephan et al; licensee BioMed Central Ltd.