Phosphorus nutrition affects temperature response of soybean growth and canopy photosynthesis

Abstract

In nature, crops such as soybean are concurrently exposed to temperature (T) stress and phosphorus (P) deficiency. However, there is a lack of reports regarding soybean response to T × P interaction. To fill in this knowledge-gap, soybean was grown at four daily mean T of 22, 26, 30, and 34◦ C (moderately low, optimum, moderately high, and high temperature, respectively) each under sufficient (0.5 mM) and deficient (0.08 mM) P nutrition for the entire season. Phosphorus deficiency exacerbated the low temperature stress, with further restrictions on growth and net photosynthesis. For P deficient soybean at above optimum temperature (OT) regimes, growth, and photosynthesis was maintained at levels close to those of P sufficient plants, despite a lower tissue P concentration. P deficiency consistently decreased plant tissue P concentration ≈55% across temperatures while increasing intrinsic P utilization efficiency of canopy photosynthesis up to 147%, indicating a better utilization of tissue P. Warmer than OTs delayed the time to anthesis by 8–14 days and pod development similarly across P levels. However, biomass partitioning to pods was greater under P deficiency. There were significant T × P interactions for traits such as plant growth rates, total leaf area, biomass partitioning, and dry matter production, which resulted a distinct T response of soybean growth between sufficient and deficient P nutrition. Under sufficient P level, both lower and higher than optimum T tended to decrease total dry matter production and canopy photosynthesis. However, under P-deficient condition, this decrease was primarily observed at the low T. Thus, warmer than optimum T of this study appeared to compensate for decreases in soybean canopy photosynthesis and dry matter accumulation resulting from P deficiency. However, warmer than OT appeared to adversely affect reproductive structures, such as pod development, across P fertilization. This occurred despite adaptations, especially the increased P utilization efficiency and biomass partitioning to pods, shown by soybean under P deficiency.