Isolation and characterization of 5'-regulatory region of mouse activin βA subunit gene

Abstract

We isolated genomic clones that contain the 5'-flanking region of the mouse activin βA subunit gene. The nucleotide sequence determination of the 5'-flanking region of the gene and the comparison of that with the reported mouse cDNA structure identified the putative 5' regulatory region, a novel first exon and a part of the first intron of the gene within this region. The putative 5' regulatory region of the mouse activin βA subunit gene directed the expression of CAT gene in transfected HT1080 cells. Successive deletions of this region demonstrated a 400-bp region that exerts a strong positive effect on promoter activity of the mouse activin βA subunit gene.