mRNA expression on Neuropeptide Y (NPY) to exercise intensity and recovery time

Abstract

[Purpose] The purpose of this study was to analyze the effect of exercise intensity and recovery times on the extension of Neuropeptide Y in mice hypothalamus. [Subjects] The mice (ICR) were housed per cage in a temperature (20-23°C) and light controlled environment with a 12:12h light-dark cycle.[Methods] ICR were divided into ten groups, 8 mice each group: rest time group, low-intensity exercise, moderate-intensity and high-intensity. The exercise was carried out on a treadmill adapted in order to permit 2 animals to run at the same. Neuropeptide Y transcript contents were estimated by RT-PCR and Real Time PCR. DNA laddering was used to detect DNA fragmentation as an indicator of Neuropeptide Y. [Results] The Neuropeptide Y mRNA relationship to exercise intensity showed significant differences immediately after exercise. The Neuropeptide Y mRNA relationship to exercise intensity showed differences on low-intensity, moderate-intensity and high-intensity. Neuropeptide Y mRNA showed differences between exercise levels of low-intensity to moderate-intensity and low-intensity to highintensity. [Conclusion] It is noteworthy that the expression of NPY mRNA can be effected by stability time and the intensity of exercise. Neuropeptide Y mRNA expression is connected with recovery times at moderate-intensity and high-intensity. The present results suggest the possibility that exercise intensity and recovery times regulated of the desire for food.