Green synthesis and charcterization of silver nanoparticles from leafs extracts of rosa indica and its antibacterial activity against human pathogen bacteria

Abstract

This work was carried out for green synthesis of silver nanoparticles and investigation of its antibacterial activity. Rosa indica is of considerable interest and is well known compound because of its antioxidant, antidibetic, anti-inflammantry, antimicrobial activities. In the present study rapid and facile synthesis of silver nanoparticles at room temperature has been shown. On addition of plant extracts to Silver nitrate solution the change in color of the reaction mixture was obsereved which proved the formation of nanoparticles. Further, the green synthesised silver nanoparticles were characterized UV-Vis spectrophotometer, XRD analysis, FTIR, DLS, SEM with EDX. The average particles size of silver nanoparticles show between 1-100 nm through DLS anaylsis. Antibacterial activity of the Silver nanoparticles was evaluated by testing against Gram negative (Pseudomonas aeruginosa) and Gram positive(Bacillus subtilis) bacteria.