Induction of heat shock protein messenger RNA in maize mesocotyls by water stress, abscisic acid, and wounding

Abstract

Exposure of the excised growing region of the mesocotyl of young corn seedlings to heat shock stimulated the production of specific heat shock proteins and the intensification of synthesis of two proteins with a molecular weight of approximately 70,000. Water stress and abscisic acid also stimulated synthesis of these 70,000-dalton proteins, and other unique proteins distinct from those induced by heat shock. Growing tissues of intact corn mesocotyls exposed to heat shock, water stress, or abscisic acid accumulated mRNA species homologous to a cloned genomic probe of the 5′ end of the 70,000-daltonDrosophila heat shock protein gene. Since cut segments of the mesocotyl under unstressed conditions produced a similar mRNA, we suggest that the hsp 70 gene is activated in corn by a variety of diverse stresses. Production of the mRNA is rapid, but transient, being induced within 3 hours of the imposition of the stress, but declining after reaching a maximum at 9 hours.