INTERGENERIC HYBRIDIZATION AMONG FIVE GENERA OF THE FAMILY LESSONIACEAE (PHAEOPHYCEAE) AND EVIDENCE FOR POLYPLOIDY IN A FERTILE PELAGOPHYCUS×MACROCYSTIS HYBRID

Abstract

Hybridization was attempted by combining gametophytes between intergeneric pairs among the following taxa in the Lessoniaceae: Macrocystis pyrifera (L.) C. Agardh, M. integrifolia Bory, M. angustifolia Bory, Pelagophycus porra (Leman) Setch., Nereocystis luetkeana (Mert.) Post & Rupr., Dictyoneurum californicum Rupr., and Dictyoneuropsis reticulata (Saud.) Smith. Hybrid sporophytes were produced in some combinations involving Macrocystis × Pelagophycus and Macrocystis × Dictyoneurum, and in all combinations of Dictyoneuropsis × Dictyoneurum. This is the first report of intergeneric hybrids involving Dictyoneurum. Gametophytes of P. porra had 16–24 chromosomes. Gametophytes from a fertile Macrocystis‐Pelagophycus hybrid were crossed with Macrocystis and Pelagophycus gametophytes. Hybrid male gametophytes and Pelagophycus female gametophytes produced sporophyte progeny, but hybrid males with Macrocystis females did not. A single hybrid female gametophyte did not produce gametophytes in combination with hybrid males, Pelagophycus males or Macrocystis males. The hybrid gametophytes had approximately 30 chromosomes. It is hypothesized that the hybrid is an alloploid, containing a complete set of Macrocystis and Pelagophycus chromosomes, which may have allowed meiosis and sporogenesis to proceed normally in the hybrid sporophyte found in the sea. Thus, reproductive isolating mechanisms appear to operate at both pre‐ and postzygotic stages, and both can be overcome in intergeneric hybrids. Copyright © 1995, Wiley Blackwell. All rights reserved