Biological effects of grape polyphenols processing products in experimental metabolic syndrome

Abstract

Objective - Biological effects of grape processing products (GPP) rich in polyphenols are still not studied completely. The aim of the investigation was to study co-interactions between peroxidation lipid oxidation (PLO) and nonspecific proteases and their inhibitors activity in the animals with the model of metabolic syndrome (MS) corrected with GPP. Material and Methods - The study was performed on 54 white male rats of the weight of 180-200 g. The animals of experimental groups were fed by the standard menu and were given 10% fructose solution instead the drinking water during 8 weeks, which promoted development of MS. “Enoant”, “Enoant-premium” and “Fenocor” were used as GPP in dosage of 2.5 ml/kg during 4 weeks just after 5 th week. The PLO was estimated according to the concentration of thiobarbituric acid active products (TBA-AP), ceruloplasmin (CC), catalase-like activity (CLA), peroxidase-like activity (PLA) and superoxide dismutase activity (SOD). Trypsin-like activity (TLA), elastase-like activity (ELA), alfa-1-antitripsin activity (ATA) and acid-stable inhibitors activity (ASA) were estimated. Results -MS demonstrated rise of TBA-AP by 50.4 % (р<0.01) and TLA by 19.5% (р<0.01), and drop of ATA, SOD, CLA, PLA in comparison with the control. The GPP (especially “Fenocor”) led to the block of pathogenetic link of MS because of suppression of PLO and proteases' activity and increase of their inhibitors. Conclusion -There is a tight connection between PLO and proteases' activity in MS. “Fenocor” could be used as MS-modified remedies acting positively on key links of MS.