Hptlc analysis of curcuma mangga val. Extracts and their immunomodulatory effects on delayed-type hypersensitivity response

Abstract

The purpose of the study was to analyze several extracts of Curcuma mangga rhizomes by HPTLC and determine their immunomodulatory effects on the Delayed-Type Hypersensitivity (DTH) response in Wistar rats. The C. mangga rhizomes were macerated with 73; 83 and 99% ethanol separately. HPTLC analysis was performed to evaluate the content of curcumin in various extracts of C. mangga. The immunomodulatory potential on DTH response was investigated by measuring the paw volume. All extracts were tested at the dose of 400 mg/kg bw. Quantitative analysis using HPTLC revealed that among the samples tested, 99% ethanol extract of C. mangga contained the highest amount of curcumin (9.94 µg/mL). All the samples tested stimulated DTH response. Amongst the extracts, 99% ethanol extract of C. mangga rhizomes demonstrated the highest stimulation on DTH response but it was not different significantly (P>0.05). However, the stimulatory activity of 99% ethanol extract of C. mangga was higher than levamisole as a positive control (P<0.05). The results indicate the presence of curcumin in C. mangga extract may contribute to its immunostimulatory activity.