Scanning force microscopy of chromatin fibers in air and in liquid

Abstract

We have adapted specimen preparation techniques of conventional electron microscopy for visualizing chromatin structures in the scanning force microscope (SFM) in air and in liquid. The beaded substructure of the nucleoprotein filament was obtained after hypotonic lysis of chicken erythrocytes and air drying, whereas supranucleosomal structures were preserved after treatment of cell nuclei with detergent. In the latter case, the nucleosomes were still distinct but appeared more condensed. A modified droplet diffusion‐spreading technique of chromatin from Namalwa cells (a human B‐lymphoid line) yielded a uniform filamentous morphology and similar fiber appearance. A reversible swelling of spread chromatin was observed upon exposure of air‐dried samples to solutions differing in salt concentrations. Copyright © 1995 Wiley Periodicals, Inc.