Mir-19a-3p functions as an oncogene by regulating fbxo32 expression in multiple myeloma

Abstract

Background: Multiple myeloma remains a virtually incurable hemato-logic malignancy, which is featured with the aberrant growth of malig-nant plasma cells. Aims: To elucidate the functions of miR-19a-3p in multiple myeloma. Study Design: Cell study. Methods: Cell counting kit-8 assay was performed to detect cell viabili-ty, and flow cytometry was conducted to detect cell apoptosis. Bioinfor-matics analysis predicted miR-19a-3p-associated biological function, pathway, core regulatory network, and target genes. Luciferase reporter assay verified the target sequence of miR-19a-3p regulating FBXO32. Results: miR-19a-3p is upregulated in multiple myeloma cells (p<0.01) and patients with multiple myeloma (p<0.001). Overexpressed miR-19a-3p significantly increased cell viability (p<0.05) and inhibited cell apoptosis (p<0.01). FBXO32 is a target gene of miR-19a-3p (p<0.01). Moreover, FBXO32 is downregulated in MM, and it significantly decreased cell viability (p<0.05) and promoted cell apoptosis (p<0.01). FBXO32 significantly rescued the influence of miR-19a-3p-inhibiting cell apoptosis (p<0.05). Conclusion: miR-19a-3p promoted cell proliferation and inhibited cell apoptosis by degrading the target FBXO32 mRNA in multiple myeloma.