Crystallization and preliminary X-ray analysis of an anti-LewisX Fab fragment with and without its LewisX antigen

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Abstract

A Fab fragment of anti-LewisX murine monoclonal antibody 291-2G3-A has been crystallized using the sitting-drop vapour-diffusion method. X-ray data sets have been collected for the native Fab fragment to 2.05 Å resolution and for the Fab fragment soaked with LewisX to 3.2 and 1.8 Å resolution.

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Van Roon, A. M. M., Pannu, N. S., Hokke, C. H., Deelder, A. M., & Abrahams, J. P. (2003). Crystallization and preliminary X-ray analysis of an anti-LewisX Fab fragment with and without its LewisX antigen. Acta Crystallographica - Section D Biological Crystallography, 59(7), 1306–1309. https://doi.org/10.1107/S0907444903010096

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