Preparation of Water Soluble Yeast Glucan by Four Kinds of Solubilizing Processes

Abstract

(1→3)-β-D-glucan from the inner cell wall of\rSaccharomyces cerevisiae is considered a member of a class of drugs known as biological\rresponse modifiers (BRM). However the glucan was an insoluble polysaccharide,\rwhich could be the major barrier to the utilization of glucan. In this case,\rthe insoluble glucan was convent into a soluble form by four kind of\rsolubilizing processes. The yield, solubility, chemistry structure and\rimmunoprophylaxis efficacy of the soluble products were compared. Our date\rsuggest that urea has a significant effect on yield, and DMSO has a significant\reffect on solubility. FT-IR spectra, 13C\rNMR spectra and helix-coil transition analysis demonstrate that the chemistry\rstructure of native and solubilizing glucans have no significant difference.\rThey still have the triple helical structure. The solubility and\rimmunoprophylaxis efficacy assay indicate that the introduction of phosphate\rgroup not only enhanced the solubility of glucan, but also improved the\rsurvival rate of mice challenged with E.\rcoli.