Preparation of Modified Agarose Gels Containing Thiol Groups.

Abstract

A simple an efficiente route for the preparetion of thiol-agarose derivatives of various degrees of thiol-groups in the agarose gel networking involves the following steps: (a) introduction of epoxide groups; (b) treatment by sodium thiosulfate to convert epoxide groups into S-alkyl-thiosulfate structures; (c) reductive cleavage of the alkyl thiosulfate structures by dithiothreitol.