Histological study of follicular atresia in the ovary of the domestic hen (Gallus domesticus)

Abstract

Semi-serial (1 in 20) sections of ovaries were studied and only two types of atresia were identified - non-bursting and bursting. Smaller, non-yolky follicles (< 1 mm diameter) showed non-bursting atresia. Atresia in follicles >1 mm diameter was invariably of the bursting type which involved the rupture of the follicular wall, and the extrusion of yolk and cellular debris through the rupture site into the stroma. However, this rupture site was small and consequently was not visible in every section but it could always be seen when the follicle was followed in semi-serial sections. The mitotic index of granulosa cells in bursting atretic follicles was much lower than that for normal follicles. The most common criteria for distinguishing non-bursting atretic follicles were the extremely shrunken, irregularly shaped oocytes and the separation of the granulosa from the theca. In bursting atretic follicles, reliable indications were the presence in the ooplasm of some cells or cellular debris, and disorganization of the yolk and granulosa tissue. The presence of pycnotic nuclei in the granulosa cells was not a consistent feature of all atretic follicles of the hen.