Raman spectroscopic study of nickel oxide nanoparticles and its antibacterial activity

Abstract

This paper illustrates the Raman Spectroscopic study of Nickel Oxide nanoparticles synthesized using ASH supported method [1]. Synthesized nanoparticles were isochronally annealed for 2 hrs at 300°C & 700°C. These particles are characterized using Raman spectroscopy & Scanning Electron Microscopy. Raman analysis is in good agreement of XRD results reported in our previous paper [2]. It confirms the formation of pure NiO cubic phase at 700°C annealing temperature. Scanning Electron Microscopy (SEM) elucidates the morphology of the particles & its size was found to be in between 40-90 nm. The antibacterial activity of the NiO nanoparticles was investigated by cup & well diffusion method using pathogenic strains including three gram negative bacteria i.e. Pseudomonas aeruginosa, Salmonella typhi, E.coli and one gram positive species i.e. methicillin-resistant Staphylococcus' aureus. Antibacterial activities were studied by measuring the diameter of zone of inhibition. The Staphylococcus aureus was found to be susceptible towards the NiO nanoparticles with diameter of zone inhibition of 26mm.