Translational Clinical Research: Use of Allogeneic Platelet-Rich Fibrin (PRF) for Wound Regeneration in Two Small-Sized Dogs

Abstract

Platelet-rich fibrin (PRF) is a recent regenerative biomaterial applied in clinical wound healing. This study aims to evaluate the safety and efficacy of allogeneic PRF therapy as a regenerative methodology for treating skin wounds in two small dogs. A Miniature Pinscher and a mixed breed dog weighing 3.6 and 9.5 kg, aged 5 and 10 years, with extensive skin wounds implicating significant tissue loss and with abundant yellow exudate were allocated to this study. Each allogenic PRF treatment was derived from screened donors and consisted of grafting PRFs at the wound recipient area. Nevertheless, the blood group of the donor or recipient was not considered. The wound from case 1 received three PRF treatments during the initial two weeks of treatment, and case 2 received four PRF treatments, followed by single weekly ones. The utilization of PRFs was suspended as soon as relevant wound contraction and epithelization were observed. Both animals received systemic antibiotics prescribed at the emergency room by the first clinician. The wounds were cleaned using sterile saline only. Two weeks later, the lesions had reduced by more than 80%. All lesions exhibited aesthetic and uneventful healing. No rejection, necrosis, or infection signs were observed. PRF acted as a regenerative biomaterial, forming vascularized granulation tissue, followed by epithelization and wound resolution.