Quantitative analysis of ethanol content in blood by gas-liquid chromatography.

Abstract

The ethanol content in blood was determined by gas-liquid chromatography using solid sampler and hydrogen flame ionizing detector. To 1 ml of blood specimen, 1∼2 mg of isopropyl alcohol was accurately added as an internal standard, and about 0.1 ml of the mixture was applied to gas chromatograph equipped with a column containing 5% glycerol coated on Celite 545. Calibration of ethanol content was performed by comparing the peak area of ethanol with that of internal standard. Recovery was almost quantitative. Change of ethanol content in blood was determined by this method and Conway method, and the results were compared. © 1967, The Pharmaceutical Society of Japan. All rights reserved.