METHYL GALLATE FROM JIRINGA (ARCHIDENDRON JIRINGA) AND ANTIOXIDANT ACTIVITY.

Abstract

Objective: This research was study about phytochemical active phenolic compound from pods of jiringa (Archidendron jiringa (Jack) I. C. Nielsen) and it’s antioxidant.Methods: Pods of jiringa were air dried and macerated with methanol. Extract was evaporated using rotary evaporator, and then, crude extract dissolved with water and partitioned with ethyl acetate. Extracts were evaporated and partitioned again with methanol and n-hexane. Column and preparative chromatography used to separate pure compound. Pure compound as methyl gallate was identificated with data from nuclear magnetic resonances of proton H (1H NMR), NMR of carbon (13C NMR), and mass spectrometry (MS). Antioxidant activity of pure compound tested by 1,1-diphenyl-2-picrylhydrazyl method.Results: We found pure phenolic compound from pods of jiringa as methyl gallate that exists on fraction III-2, and it has high antioxidant activity with inhibition concentration 50 was 3.7576 μg/ml.Conclusions: Pods of jiringa contain of active phenolic compound as methyl gallate that has high antioxidant activity. Therefore, it can be used as a source of natural antioxidant.