In Vitro Biological Control of Fungus Botrydiplodia sp. Using Plant-based Pesticide

Abstract

Botryodiplodia sp. is a pathogenic fungus with a wide range of hosts, including agricultural and forestry crops. Botryodiplodia sp. attacks have been reported to cause symptoms of dry rot, branch rot, fruit rot, and shoot death resulting in decreased productivity. Efforts to control diseases caused by the fungus Botryodiplodia sp. are needed to avoid further infection in healthy plants. Control using chemical pesticides will cause several negative problems for the environment and human health. Alternative control of Botryodiplodia sp. is using biopesticide. This study aimed to analyze the ability of plant extract-based pesticides to inhibit the growth of Botryodiplodia sp. in vitro. The method used was the food poisoning method on PDA and PDB with concentrations of biopesticide 0%, 0.25%, 0.5%, 1%, 4%, 7%, and 10%. The result of this research is the concentration of 0.5% is sufficient to inhibit the growth of Botrydiplodia sp by 11.73% in PDA and 21.96% in PDB. Biopesticides are suspected to have fungistatic characteristics so that they only inhibit but do not stop fungal growth.