Induction of Macrophage Cytotoxicity by Slime Products Produced by Encapsulated Lactococcus lactis ssp. cremoris.

Abstract

Induction of macrophage (Mφ) cytotoxicity by capsular slime product (s) by Lactococcus lactis subsp. KVS 20 was examined cremoris in vivo and (CSP) produced (PE-Mφ) was significantly in vitro. macrophages The cytotoxic activity of peritoneal of a dose of After the injection of CSP in vivo. injection induced by the intraperitoneal 100mg/kg, the activity rapidly increased from day 3 and reached a maximum of 65% on day 5. Further, CSP enhanced the proportion of Fcγ-positive Mφ in the peritoneal cavity. When the culture supernatants of PE-Mφ which was stimulated with CSP in vivo (CSP-Mφ-Sup) were cells was extremely S-180 cells. However, co-cultured inhibited, PE-Mφ with Sarcoma-480 (S-180) indicating that CSP-Mφ-Sup cells, the growth are directly cytotoxic of tumor against stimulated with CSP in vitro did not exhibit any cytotoxicity cytotoxic S-180 cells. against stimulated factor (s) when These results with CSP produces that PE-Mφ strongly suggest in vivo, and the cytotoxic activity of PE- Mφ may be enhanced by the factor (s). The action of lymphoid cells involved in the induction of cytotoxic Mφ by CSP is discussed. Anim.