Effect of ceftriaxone and timentin antibiotics on morphogensis in the in vitro culture of bread wheat triticum aestivum L

Abstract

Aim. To study the influence of antibiotics Ceftriaxone and Timentin on the morphogenic calli formation shoots regeneration and roots formation in two bread wheat varieties Triticum aestivum L., to study the effect of Ceftriaxone on the growth dynamics of plants. Methods. In vitro plant tissue culture, statistical methods: analysis of variance and correlation and regression analysis. Results. The influence of β-lactam antibiotics Ceftriaxone and Timentin on morphogenesis (morphogenic calli formation, shoots regeneration and rhizogenesis), was studied in apical 18-day-old wheat calli. Two wheat genotypes of with a different growth pattern, winter and winter-spring, were used. Conclusions. Timentin and Ceftri-axone affected morphogenesis in the bread wheat apical calli by stimulating morphogenic calli formation; Ceftriaxone additionally accelerated the appearance of the meristematic zones in the initial calli. The roots formation depended primarily on the wheat genotype, independently of an antibiotic applied. The presence of Ceftriaxone in the culture medium stimulated rooting and growth of regenerating plants as well as their biomass increment.