IL-6 prevents CXCL8-induced stimulation of EpCAM expression in ovarian cancer cells

Abstract

Epithelial cell adhesion molecule (EpCAM), which is expressed in the majority of epithelial tissues, exhibits tumor growth promoting abilities and is overexpressed in human epithelial ovarian cancer. Therefore, EpCAM is considered to be a promising target for specific immune-based therapies. The present study evaluated the role of IL-6 and IL-8 in the expression of EpCAM in the A2780 human ovarian cancer cell line. Furthermore, the cellular localization of the EpCAM protein in A2780 cells was determined and the effect of EpCAM inhibition on the proliferation of the A2780 cells was investigated. An MTT assay demonstrated that blocking EpCAM with anti-EPCAM antibodies had no effect on cellular metabolic activity (proliferation). Gene expression analysis revealed that IL-8 increased EpCAM expression, whereas IL-6 and the combination of IL-6/IL-8 had no effect on EpCAM expression. Immunofluorescence analysis confirmed that EpCAM is expressed on A2780 cell membranes. The present results demonstrated that IL-8 increased EpCAM expressionatthemRNAlevelinovariancancercellsandsuggested apotentialroleofIL-6asaninhibitorofIL-8-stimulatedEpCAM expression.