Extraction and purification of bromelain enzyme from queen pineapple

Abstract

The purpose of this study is to produce bromelain powder from extract of pineapples. Bromelain enzyme extracted by enzyme isolation method then purified by ammonium sulphate salt, ethanol and ultrafiltration membranes. Then filtering and drying is done using a vacuum dryer. The variables of ammonium sulfate salt concentrations are 10%, 15%, 20%, 25%, and 30%. The properties and bromelain activity of the pineapple extract was determined. The best result of bromelain enzyme powder from pineapple were precipitated by ammonium sulphate 15%, and ethanol 5% using membranes with activity enzyme are 0,658 U / gr and protein content were 2,03%.