Expression, purification, crystallization and preliminary crystallographic analysis of spermidine acetyltransferase from Escherichia coli

Abstract

The spermidine acetyltransferase (SAT) from Escherichia coli catalyses the transfer of acetyl groups from acetyl-CoA to spermidine. SAT has been expressed and purified from E. coli. SAT was crystallized by the sitting-drop vapour-diffusion method to obtain a more detailed insight into the molecular mechanism. Preliminary X-ray diffraction studies revealed that the crystals diffracted to 2.5Å resolution and belonged to the cubic space group P23, with unit-cell parameters a = b = c = 148.7Å. They contained four molecules per asymmetric unit. © 2013 International Union of Crystallography.