Spreading Phenomena of Mobile Colistin Sulfate Resistant (Mcr-1) in Broiler Chickens and its Residue In Chicken Meat

Abstract

Colistin is considered the lastly used antimicrobial drug for the treatment of many infections. As the ex-tensive use of colistin, resistance has been increasing rapidly, thus, 200 different chicken samples were examined for isolation of E. coli and for colistin resistance gene. Eighty-three selected samples (chicken breast) were subjected to detection of colistin residues by HPLC (High-performance liquid chromatography). In this research, we used poly-merase chain reaction (PCR) for the detection of the mcr-1 gene, and phylogeny was performed on three isolates to detect mcr-1 gene mutations and relationships. The percentage of isolated E. coli was 25.5%. All isolates showed resistance to colistin in disc-diffusion assay, while in MIC (minimum inhibitory concentration) method 68.8% exhibited resistance. Colistin was recorded as 33.7% in chicken breast by HPLC. Furthermore, mcr-1gene was detected 54% using PCR. Sanger sequencing revealed the same identity (100%) between the three examined strains despite coming from different sources. It could be concluded that the practices inside poultry farms might be a possible source of spread of antimicrobial resistance to the food chain in Egypt.