hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells. © 2009 by the University of Wrocław.
CITATION STYLE
Zhu, X., Yang, N., Cai, J., Yang, G., Liang, S., & Ren, D. (2010). The intrabody targeting of hTERT attenuates the immortality of cancer cells. Cellular and Molecular Biology Letters, 15(1), 32–45. https://doi.org/10.2478/s11658-009-0032-2
Mendeley helps you to discover research relevant for your work.