A Novel Fingerprinting Method for Glycosides in Nakshatra Plants by HPTLC

Abstract

Nakshatra plants are medicinally important and used from olden days onwards to treat various diseases. In the present study, a HPTLC method was developed for sepn. of glycosides in plants. The powd. drug of Nakshatra plant was extd. with ethanol and identification was done chem. by Molisch's, Fehling's, Bial's test and sepn. of glycosides was performed densitometrically at λ = 254-700 nm using as a mobile phase, Et acetate: methanol: water (20:2.5:2.5). The results of qual. anal. revealed the presence of carbohydrates, reducing sugars and pentose. HPTLC method achieved a good sepn. of glycosides at 254 nm, 304 nm, 354 nm, 404 nm with recorded peak areas and Rf values. The comparative common compd. of sample glycosides was also done. [on SciFinder(R)]