Screening of indigenous Rhizobacteria isolates from Patchouli Rhizosphere producing HCN, Siderophores and Chitinolytic Enzymes

Abstract

The consortium of bacteria that inhabit the rhizosphere, acting as plant growth promoting rhizobacteria is known as indigenous rhizobacteria. Rhizobacteria can produce chemical compounds that can protect plants from pathogens and various plant-disturbing organisms. Optimization of the growth and yield of patchouli can be done by utilizing the potential of rhizobacteria derived from patchouli rhizosphere. This study aims to obtain rhizobacteria capable of producing secondary metabolites of HCN compounds, siderophores and chitinolytic enzymes which are thought of as biocontrol agents in plant protection. The soil samples used were taken from patchouli rhizosphere areas in three locations, namely Alue Abed, Aceh Jaya and Krueng Itam and Purwosari, Nagan Raya. The exploration results obtained 4 isolates of rhizobacteria from Alue Abed, Calang, 8 isolates from Krueng Itam, Nagan Raya and 37 isolates from Purwosari, Nagan Raya. Based on the test results of qualitative and quantitative physiological properties, 34 isolates were able to produce HCN, 49 isolates produced siderophores and 42 isolates were able to carry out chitinolytic enzyme activity. Rhizobacteria isolate producing HCN, siderophores and chitinolytic enzymes are potential indigenous rhizobacteria candidates and potential for future testing as bio-protections of plants from pathogen attack.