Chronic Gαs signaling in the striatum increases anxiety-related behaviors independent of developmental effects

Abstract

Current research in the field of anxiety disorders is largely receptor-centric, leaving intracellular pathways largely unexplored. Gαs, the G-protein which stimulates adenylyl cyclase and L-type voltage-gated calcium channels, may be one intracellular molecule regulating anxiety-related behaviors as increased efficacy of Gαs signaling has been noted in patient populations that suffer from anxiety. We report here anxiety-related behaviors in two lines of transgenic mice expressing a constitutively active isoform of Gαs (or Gαs*). The first line expressed Gαs* throughout postnatal forebrain neurons, while the second line of mice conditionally expressed Gαs* selectively in the striatum (Gαs*str mice). In the open field, both lines of mice showed a significant preference for the periphery suggesting that expression of Gαs* in the striatum alone was sufficient to produce an anxiogenic phenotype. In the light/dark box, Gαs*str mice exhibited longer latencies to enter the light and spent significantly less time in the lit compartment. Similarly, Gαs*str mice showed longer latencies to enter the open quadrants and spent less time in the open quadrants of the elevated zero maze. Interestingly, these anxiety-related phenotypes were largely unrelated to developmental effects as mice expressing the Gαs*str transgene during development, but not at testing, were normal on most measures. These observations show that chronic Gαs signaling in the striatum is sufficient to trigger anxiety-related behaviors largely independent of developmental effects and suggest the cAMP pathway or L-type voltage-gated calcium channels may be viable targets for future pharmacological intervention in the treatment of anxiety disorders. Copyright © 2008 Society for Neuroscience.