Biotransformation of Rutin to Quercetin by Microorganisms

Abstract

Quercetin is a kind of flavonoid drugs, which is produced mainly by direct extracting from Flos Sophorae or acid hydrolysing from rutin. In this study, microorganism strains that can transfer rutin to quercetin were isolated from soil, screened by TLC and re-screened by HPLC. As a result, strain D97 with the highest transformation ability was obtained. The hydrolyze enzyme was intracellular enzyme and transformation rate was 75.6% within a system comprising of 50mg wet cells in 4mL of 0.3% rutin solution and transformed for 72hr at 25°C. Strain D97 was identified as Pseudomonas sp. by integrating the 16S rDNA phylogeny analysis result and a variety of morphological, physiological and biochemical features.