Protective effect of nano-selenium and ionized selenium against the testicular damage, endocrine disruptor and testicular ultrastructure of bisphenol a in albino male rats

Abstract

Objective: The current study was conducted to investigate the protective effect of selenium preparations against the reproductive toxicity of bisphenol A. Materials and Methods: Sixty adult Albino male rats (aged from 2-3 months and average weight 150-180 g) were used and they were randomly divided into sex equal groups. Group I served as control, group II were orally administered BPA in a dose level of 1/10 LD50 (150 mg kg−1 b.wt.) for 8 successive weeks, group III were gavaged with BPA plus ionized selenium in a dose level of (3 mg kg−1 b.wt.), group IV animals in this group orally given BPA plus nano-selenium particles in a dose level of (2 mg kg−1 b.wt.) and groups V and VI were gavaged with both IS and NSP with previous doses. All data were analyzed using one way analysis of variance (One way ANOVA) followed by Duncan test using SPSS version 16.0 statistical software. Results: The obtained results revealed that exposure to BPA significantly induce testicular damage observed in testicular weight, sperm morphology and significant decrease in testosterone level and increase in percent of sperm abnormalities. Ultrastructural examination of testis revealed that both selenium preparations reduced the testicular damages induced by BPA. Conclusion: High concentrations of Se in testis and epididymes indicate its importance for the processes of production and maturation of spermatozoa. Nano-Se has lower toxicity and possesses equal efficacy in increasing the activities of selenoenzymes. It can serve as an antioxidant with reduced risk of Se toxicity and a potential chemopreventive agent if the induction of GST by Se is a crucial mechanism for its chemopreventive effect.