Isolation and characterization of thermophilic bacteria as amylase enzyme produced by hots spring in rianiate samosir, indonesia

Abstract

Thermostable enzymes (such as amylase) are enzymes with great potential to overcome industrial technical problems associated with high-temperature processes. This type of enzyme can be isolated from thermophilic bacteria obtained from hot springs, such as hot springs Rianiate, Samosir. The objectives of this study were to obtain isolates, morphological and biochemical characterization, followed by determining the potential of selected isolates qualitatively. Hot water samples were determined by purposive sampling from two location points, with temperatures 40 °C, 43 °C and 45 °C with a pH of 4. Isolation of thermophilic bacteria using Nutrient Agar (NA) media. There were 12 thermophilic bacterial isolates from two sample locations isolated with the distribution of 2 isolates at 40 °C and 10 isolates at 43 °C, while at 45 °C there were no isolates. The twelve isolates were able to produce an amylase enzyme. The amylase enzyme production was determined by the presence of a clear zone around the colony on the starch agar medium after being dripped with iodine solution. The three isolates that produced the largest clear zone were sp1(2), sp3(1) and sp6, namely 83.1 mm, 79.9 mm and 80.2 mm, respectively. A biochemical test was carried out on isolates with the largest clear zone diameter value. The characterization results showed that the isolate was predicted to belong to the genus Basillus sp. These results indicate that the thermophilic bacteria from the hot springs of Rianiate, Samosir have the potential to produce amylase enzymes.