In vitro anti-inflammatory activity of aqueous, ethanolic and ethereal extracts of rhizomes, leaves and stems of Anredera vesicaria

Abstract

Anredera vesicaria has been used traditionally in the eastern region of Cuba to treat various inflammatory conditions. No reports of the anti-inflammatory activity of this species have been published so far. The aim of this study was to determine the in vitro anti-inflammatory activity of the aqueous, ethanolic and ethereal extracts of the leaves, stems and rhizomes of the A. vesicaria. Serial dissolutions were prepared at 125, 250 and 500μg/mL from each dry extract of the plant parts, which were subjected to determinations of the anti-inflammatory activity in vitro by Human Red Blood Cell (HRBC) membrane stabilization method, using diclofenac sodium as a reference drug. All extracts were able to stabilize the erythrocyte membrane in hypotonic solution and exhibited major activity than diclofenac sodium at different doses. The stems extracts showed the highest activity, which suggest a major concentration of compounds with potential anti-inflammatory activity.