Photoinhibition in benthic diatom assemblages under light stress

Abstract

Microphytobenthos are frequently subjected to light intensities higher than those required to saturate photosynthesis, which consequently can cause photoinhibition. Photo - system II (PSII) protein D1 (the main target of photoinhibition) and xanthophyll cycle pigments were quantified in epipelic benthic diatom assemblages under high irradiance, in the presence of inhibitors and promoters of photoprotection mechanisms. Levels of D1 protein were significantly lower under high irradiance (1 h, 1500 μmol photons m -2s-1) than under low light (80 μmol photons m -2s-1), corresponding to a photoinhibition of 22 to 29%. Photoinhibition increased to 44 and 80% in the presence of lincomycin (inhibitor of chloroplast-protein synthesis) and dithiothreitol (inhibitor of the xanthophyll cycle), respectively. High light treatment had no significant effect on D1 protein concentrations in the presence of added glutathione and ascorbate, scavengers of reactive oxygen species (ROS). In contrast, the ROS promoter methylviologen increased photoinhibition to 63%. Under light stress, the functional stability of PSII reaction centres of the studied epipelic benthic diatoms was more dependent on xanthophyll cycle activation than on D1 protein recycling mechanisms, and our results substantiate the role of antioxidants in photoprotection via ROS scavenging. © Inter-Research 2013.