Association genetics of complex traits in plants

Abstract

Summary 909 I. Introduction 910 II. Genotyping 910 III. Phenotyping 911 IV. Study designs 912 V. The genetics of the 'omics' 912 VI. Missing heritability: the dark matter of the genome 913 VII. Gene interactions 914 VIII. Many rare alleles 914 IX. Looking in the wrong place 914 X. Looking but not seeing 915 XI. Needles in a haystack 915 XII. Confounding effects 916 XIII. Replicating and verifying associations 916 XIV. The genetic architecture of quantitative traits in plants 917 XV. Outlook 918 Acknowledgements 919 References 919 +Summary: Association mapping is rapidly becoming the main method for dissecting the genetic architecture of complex traits in plants. Currently most association mapping studies in plants are preformed using sets of genes selected to be putative candidates for the trait of interest, but rapid developments in genomics will allow for genome-wide mapping in virtually any plant species in the near future. As the costs for genotyping are decreasing, the focus has shifted towards phenotyping. In plants, clonal replication and/or inbred lines allows for replicated phenotyping under many different environmental conditions. Reduced sequencing costs will increase the number of studies that use RNA sequencing data to perform expression quantitative trait locus (eQTL) mapping, which will increase our knowledge of how gene expression variation contributes to phenotypic variation. Current population sizes used in association mapping studies are modest in size and need to be greatly increased if mutations explaining less than a few per cent of the phenotypic variation are to be detected. Association mapping has started to yield insights into the genetic architecture of complex traits in plants, and future studies with greater genome coverage will help to elucidate how plants have managed to adapt to a wide variety of environmental conditions. © 2010 The Authors. New Phytologist © 2010 New Phytologist Trust.