Evaluation of Mueller-Hinten agar for disk diffusion susceptibility tests

Abstract

Twelve lots of commercially perpared Mueller-Hinton agar from four medium manufacturers were evaluated for performance with recommended quality control organisms, medium pH, agar depth and uniformity, and characteristics of the zones of inhibiton. Only 2 of 12 lots were acceptable for disk diffusion susceptibility tests. Significant problems were observed with the preparation and pouring of the agar media.