Mkl1-dependent gene activation is sufficient to induce actin cap assembly

Abstract

Actin-dependent forces mechanically control both the position and shape of the nucleus. While the mechanisms that establish nuclear position are well defined, less understood is how actin filaments determine nuclear shape. We recently showed that nuclear envelope-spanning LINC complexes promote stress fiber assembly by activating the small GTPase RhoA and Mkl1-dependent gene activation. We now report that a subset of these stress fibers associate with the apical face of the nuclear envelope through LINC complexes that contain the inner nuclear membrane protein Sun2. Apical stress fibers have previously been shown to specifically couple cell and nuclear morphology, suggesting that LINC complexes influence nuclear shape in part by regulating the small GTPase RhoA.