Rapid identification of chikungunya and dengue virus by a real-time reverse transcription-loop-mediated isothermal amplification method

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Abstract

Both Chikungunya and Dengue virus belong to the acute arthropod-borne viruses. Because of the lack of specific symptoms, it is difficult to distinguish the two infections based on clinical manifestations. To identify and quantitatively detect Chikungunya and Dengue viruses, a real-time accelerated reverse-transcription-loop-mediated isothermal amplification (RT-LAMP) platform was developed, and 26-confirmed RNA samples, 42 suspects, and 18 healthy serum samples were evaluated by the method. The RT-polymerase chain reaction (PCR) and cDNA sequencing were used as references. The results showed that it could identify the Chikungunya and Dengue virus RNA correctly in all antibody-positive samples within 1 hour, without any cross-reactions. The virus load of the positive samples was quantitatively detected with a turbidimeter. The sensitivity was 100% and specificity was 95.25%. The findings indicate that the RT-LAMP is an effective method for rapid quantity detection of Chikungunya virus and Dengue virus in serum samples with convenient operation, high specificity, and high sensitivity. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.

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APA

Lu, X., Li, X., Mo, Z., Jin, F., Wang, B., Zhao, H., … Shi, L. (2012). Rapid identification of chikungunya and dengue virus by a real-time reverse transcription-loop-mediated isothermal amplification method. American Journal of Tropical Medicine and Hygiene, 87(5), 947–953. https://doi.org/10.4269/ajtmh.2012.11-0721

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