Induction of ARI12 upon broad band UV-B radiation is suppressed by UVR8 and cryptochromes

Abstract

ARI 12 belongs to a family of 16 potential E3 ligases in Arabidopsis and is strongly induced in leaves upon low and high fluence rates (HFR) of UV-B. We have shown that ARI12 is a downstream target of the UV-B receptor, UVR8, and the transcription factors HY5 and HYH under low fluence rates. However under HFR of broad band UV-B ARI12 expression was still downstream of HY5 and HYH but increased in uvr8 mutants. To determine if other photoreceptors are responsible for the induction of ARI 12 we quantified its expression in double mutants of the UV-A and blue light receptors, CRY1/2 and PHOT1/2, and the red light receptors PHYA/B. While the expression of ARI 12 was increased in cry1/2 it was unaffected in phot1/2 and phyA/B. Therefore ARI12 expression is suppressed by UVR8 and cryptochromes, and independent of phototropins and phytochromes A and B upon HFR of broad band UV-B.