Studies on the propagation of fritillaria camtschatcensis ker-gawl. by In vitro culture. II. Effect of plant hormones on the growth, leaf emergence, and rooting of bulblets

Abstract

Bulblets isolated from small globule-scale of Fritillaria camtschatcensis Ker-GawI. were cultured on MS medium containing plant hormones under light of 1,500 lx at 25 °C for 8 weeks. NAA, kinetin and 24-epibrassinolide (EB) were tested for their effects on growth, leaf emergence, and rooting of bulblets. With individual plant hormones, EB at 0.01ppm enhanced bulblet growth and rooting, whereas 0.1ppm kinetin was optimum for advancing leaf emergence. With respect to combinations of plant hormones, that consisting of 1.0ppm NAA and 0.1ppm kinetin was optimum for bulblet growth and leaf emergence, whereas 1.0ppm NAA, 0.1ppm kinetin and 0.01ppm EB promoted rooting of bulblets.