Pharmacological Evaluation of Chrozophora tinctoria as Hepatoprotective Potential in CCl4 Induced Liver Damage in Rat

Abstract

Chrozophora tinctoria is a monoecious shrubby herb traditionally used as food supplement and in the treatment of various infectious diseases. Leaves were shown high content diterpenes, flavonoids and chromone glycosides possessing antioxidant potential. The study was designed to evaluate hepatoprotective effect of Chrozophora tinctoria leaves in carbon tetrachloride (CCl4) induced liver damage in Wistar rats and to validate its traditional claims. Thirty Wistar rats (180-200 g) of either sex were randomly divided into 5 groups (n = 6) such as Group 1 served as the normal control (0.9% Saline); Group 2 rats were administered with CCl4 1.5 mL kg-1 i.p. prepd. in 20% olive oil for induction of hepatotoxicity, Group 3 and 4 rats were orally administered with the hydro-alc. leaves ext. of C. tinctoria at a low dose 50 mg kg-1 and a high dose 100 mg kg-1, resp.; Group 5 rats treated with Silymarin 25 mg kg-1 orally as std. drug for a period of 28 days. At the end of the study, the blood samples were collected for biochem. parameters in liver, antioxidant anal. and the liver tissue for histol. anal. The CCl4 treated rats developed hepatotoxicity that evident by the elevated level of AST, ALT, ALP, bilirubin, proteins, albumin and the antioxidant enzymes like CAT, SOD, GGT and GST. Treatment with hydro-alc. leaves ext. of C. tinctoria 50 mg kg-1 shows less significant (**p<0.05) improvement, while the higher dose of C. tinctoria 100 mg kg-1 shows highly significant (**p<0.05) improvement in AST, ALT, ALP, bilirubin, proteins and albumin against disease control and almost comparable to normal and std. control. The hydro-alc. ext. of C. tinctoria 100 mg kg-1 protect liver injuries due to the presence of potent phytoconstituents and antioxidant enzymes. [on SciFinder(R)]