A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome

Abstract

Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A Watson—Crick G—C pair between G2252 in a conserved hairpin loop of 23S rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis. © 1995, Nature Publishing Group. All Rights Reserved.