Study the Interaction between Gold Nanoparticles and Bovine Serum Albumin: Spectroscopic Approach

Abstract

Interaction between gold nanoparticles of 40 & 60 nm with BSA has been investigated using UV-absorption spectrophotometry, fluorescence spectroscopy and FT-IR spectroscopy. UV-absorption spectrophotometry showed an increase in the absorption intensity with increasing the molecular ratios of gold nanoparticles of both sizes to BSA, it is found that the value of the binding constant is estimated to be 0.888 × 104 M-1 for gold nanoparticles (40 nm)- BSA complexes, and 1.16 × 104 M-1 for gold nanoparticles (60 nm)-BSA complexes. FT-IR spectroscopy analysis showed that intensities of gold nanoparticles of both sizes-BSA complexes decrease as the concentration increased.