Validated HPTLC method for quantitative determination of gallic acid in Stem Bark of Myrica esculenta Buch.-Ham. Ex D. Don, Myricaceae

Abstract

A simple, rapid, and precise HPTLC method was developed for quantitative estimation of gallic acidin stem bark of Myrica esculenta, family Myricaceae. Separation was performed on silica gel 6OF254 HPTLC plates using toluene-ethyl acetate-formic acid-methanol (3 + 3 + 0.6 + 0.4, v/v/v/v) mobile phase for separation of the extracted components. The determination was carried out in the UV densitometric absorbance-reflection mode at 280 nm. The amount of gallic acid in free and combined form in the stem bark powder was found to be 0.276 and 0.541%, respectively, on a dry weight basis. The method was validated in terms of linearity, accuracy, precision, and specificity according to International Conference on Harmonization guidelines. Gallic acid response was found to be linear over a broad concentration range of 0.4-2.0 μg/band. LOD and LOQ were 0.103 and 0.312 μg/spot, respectively. The developed method is capable of quantifying amounts of gallic acid in stem bark powder of M. esculenta.