Evaluation of commercial latex slide test for identifying Escherichia coli 0157

Abstract

A commercial latex slide test (Oxoid DR 620) for the identification of Escherichia coli 0157 was compared with a standard tube agglutination method using E coli 0157 antiserum (Difco). Thirty isolates previously confirmed as E coli 0157 were positive by both methods: 30 isolates of E coli of serogroups other than 0157, 30 isolates of non-sorbitol fermenting organisms from various genera, and one isolate of Ehermanii were all negative by both methods. It is concluded that the latex slide test offers a rapid and economical alternative to tube agglutination for the identification of E coli 0157.