Genome comparison of the Matricaria chamomilla L. varieties by the chromosome C- and OR-banding patterns

Abstract

The comparison of chromosomes stained by the modified C- and OR-banding techniques in karyotypes of three varieties of Matricaria chamomilla L. was carried out by image analysis. Nine pairs of the C- and OR-banded chromosomes of camomile were classified according to their banding patterns, sizes and centromeric indexes in karyotypes of varieties Azulena, Sibirskaya bizabololnaya and Podmoskovnaya. The correspondence between two banding patterns in homologous chromosomes of M. chamomilla L. was revealed and their generalized C- and OR- Mch genome idiograms were developed. The chromosome intra- and intervariety C-band polymorphism was detected. The autotetraploid Podmoskovnaya variety karyotype occupies an intermediate position between the karyotypes of the diploid varieties. The observed differentiation of two identical Mch genomes in Podmoskovnaya karyotype is rather regular and may be a consequence of their interaction in the course of polyploidization. The OR- chromosome banding patterns in karyotypes of all varieties were similar and no OR-band polymorphism was detected by quantitative analysis. The absence of chromosome rearrangements and similarity of C- and OR- chromosome banding patterns in the karyotypes of studied camomile varieties indicate that their chromosomes belong to one common genome in the species M. chamomilla L. © 2001 Taylor & Francis Group, LLC.