Aktivitas Antibakteri Ekstrak Lipid Sargassum polycistum Terhadap Bacillus cereus dan Staphylococcus aureus

Abstract

The macroalgae or seaweed is a biological resource of the Indonesian sea which contains the bioactive compounds. The antimicrobial resistance is a main problem This study aim to know the antibacterial activity from the lipid crude extract of Sargassum polycistum in methanol and chloroform phase in inhibiting the growth of Bacillus cereus and Staphylococcus aureus. The lipid extraction used soxhletation with methanol:chloroform solvent combination (1:2/v/v). Antibacterial assay used disc diffusion method according to Kirby-Bauer. The most lipid extraction result is obtained in methanol phase, approximately 11,2% (v/b) while its chloroform phase is only 3,8% (v/b). The chloroform phase has no inhibition zone in Bacillus cereus and Staphylococcus aureus growth while its methanol phase gives antibacterial activity where its clear zone diameter is 14 mm.