Production of extracellular protease by Streptomyces albidoflavus

Abstract

Production of protease by Streptomyces albidoflavus isolated from laterite soil was studied under submerged fermentation. The strain started protease production after 24 h of incubation and maximum level of enzyme production was found with 72 h old culture. Attempts were made to optimize the cultural conditions for getting high yields of enzyme. The optimum levels of pH and temperature for enzyme production were 7.0 and 35°C respectively. Among carbon sources, maltose (1%) supported maximum production of protease followed by trehalose, glycerol, starch and glucose. High yield of protease was recorded in the medium supplemented vdth peptone (0.75%) followed by beef extract, casein, yeast extract tryptone and NaNO3. © 2008 Academic Journals Inc.