Development of a reverse transcription-quantitative PCR assay for detection of salivirus/klassevirus

Abstract

A broadly reactive and highly sensitive reverse transcription-quantitative PCR assay to detect salivirus/klassevirus was developed. By means of the developed assay, salivirus/klassevirus was detected in 13 (93%) raw sewage, 4 (29%) secondary-treated sewage, and 9 (16%) river water samples, with a maximum concentration of 9.7×106 copies/liter. © 2013, American Society for Microbiology.